Describe the steps of eukaryotic mRNA processing from the en

Describe the steps of eukaryotic mRNA processing from the end of transcriptions until it is completely processed and ready for translation. A protein is 300 amino acids long. Which is the number of nucleotides in the section of DNA that codes for this protein? (Remember: DNA is double-stranded.) A short segment of an mRNA molecule is shown below. The polypeptide it codes for is also shown: 5\'-AUGGUGCUGAAGAUU3\' What would be the sequence of amino acids encoded by this sequence? Assume that a mutation in the DNA occurs so that the 10th base (counting from the 5\' end) messenger RNA now reads U rather than A. What sequence of amino acids will the mRNA code for? We discussed several diseases that are due to a protein with an incorrect structure. describe how the misshapen protein leads to disease.

Solution

10. RNA processing

In Eukaryotes, transcription and translation take place in the different compartment transcription takes place inthe nucleus whereas translation takes place in the cytoplasm. In prokaryotes, transcription of mRNA and translation occurs simultaneously. Thus mRNA molecules undergo little or no modification after synthesis by RNA polymerase in Prokaryotes. Eukaryote very extensively process pre mRNA destined to become RNA. The primary transcript of an RNA polymerase is refered to as pre-RNA. Processing of eukaryotic pre-mRNA involve 5 prime capping,3 prime cleavage/ Polyadenylation, splicing and RNA editing before being transported to the cytoplasm where they are translated by ribosomes.

11. 300 Amino acids code by 900 bp. Amino acid code by triplet of base.

12. the Sequence of the Amino acid encoded by that sequence will be (M V L K I) if 10th position A will change in U then the sequence will be M V L Stop that will cause non sence mutation.

13. Misfolded protein form aggreagte which causing many disease like prions and alzeimer.