Order the following regions of a gene from the most upstream

Gene Structure the basic unt of genetic nformaton also defined as the nucec acid sequence that codes for nuceatides with a fixed start point and end point codors are found in mRNA and code for sngie amno The unexpressed regions of spla genes are The strand of DNA for a hat copied by the RNA Pehrmerate to form mRNA is caled can attach to the mRNA and begin translation mven through transcripwion has and some plants b, fungi molecules deliver amino acids to ribosames during Messenger RNA (mRNA) al RNA (rRNA) d. Transfer RNA (tRNA) 11.The genetic code is said to be because more than one codon will specify a particular amino c, multiplicative 12. During translation elongation, which event happens last? nslocates from the P site to the Esite. tRNA molecule tra b. A peptide bond forms. c. A tRNA carrying an amino acid binds to the Asite. d. A tRNA molecule translocates from the A site to the P site 13. Which of the following is true about the structure of DNA? a. Purine and pyrimidine bases are joined by phosphodiester bonds. b. Purine and pyrimidine bases are attached to the 1\'-carbon of the deoxyribose sugars. c. Adjacent bases are stacked on top of each other, one base pair every 0.34 nm. d. All of the choices are correct. order the following regions of a gene from the most upstream region 1)to the most downstream region (5) #14 -35 consensus sequence 5 rut site #16 T Pribnow box

Solution

A gene has various parts in the following order (starting from the upstream region of the gene towards the downstream region)

1. the -35 consensus region

The promoter region of prokaryotes has 2 conserved regions at the -35 and the -10 positions upstream from the actual transcripton start site. The sequence at the -35 position generally consists of the sequence TTGACA and is called the -35 element. The presence of this sequence causes a very high transcription rate of that gene.

2. The Pribnow Box

This is the conserved region which is also called the -10 element and has a sequence of 5\'TATAAT3\' and is absolutely essential to start transcription in prokaryotes..In eukaryotes, it is called the TATA box and lies around 25 bases upstream to the transcription start site.

3. The coding sequence

The promoter sequence is followed by the coding sequence. In prokaryotes, the coding region can have sequences encoding multiple proteins where is in eukaryotes, there will be exons alternated by introns, which form the coding region of the gene.

4. The rut site

The rut or rho utillization site is the initial binding site for the rho protein which is rich in cytosine. Rho binds here, gets activated and uses its ATPase activity to slide down the RNA-DNA complex and finally catch up to dislodge the RNA from the DNA.

5. The terminator

The terminator is usually found towards the end of the gene, after the coding region and downstream of the Rho utilization site.