You grow a generalized transducing phage on E coli with the

You grow a generalized transducing phage on E. coli with the phenotype bio+ his - trp+ and mix it with a recipient that is bio- his + trp-. You obtain the data below: (attached)

What is the bio+ his- cotransduction frequency? Give your answer as a % and round to the nearest whole number. Do not enter a % sign in your answer, enter only the number.

What is the bio⁺ trp⁺ cotransduction frequency? Give your answer as a % and round to the nearest whole number. Do not enter a % sign in your answer, enter only the number.

What gene orders are possible? Indicate all possibilities.

Select all that apply:

B. bio trp his

C. his bio trp

You grow a generalized transducing phage on E coli with the phenotype bio his trp an mix it with a recipient that is bio his trp. You obtain the data below: Possible strain phenotypes: Actual number: bio his T trp 100 25 bio his trp 2 bio his trp 25 bio his trp what is the bio his cotransduction frequency? Give your answer as a and round to the nearest whole number. Do not enter a sign in your answer, enter only the number

Solution

(1) The bio+ his- cotransduction frequency is 100% of 125/152 = 82

(2) The bio⁺ trp⁺ cotransduction frequency is 100% of 102/152 = 67

(3) The double-crossover gametes have the lowest frequency.

bio+ his+ trp+ < bio+ his+ trp- < bio+ his- trp+ < bio+ his- trp-