In the pBR322 plasmid below which of the following does not

In the pBR322 plasmid (below), which of the following does not corresponds to a site that a specific restriction enzyme will recognize and cleave? A. Pstl B. Pvull C. tet^R D. BamHI E. EcoRI

Solution

C tetra.

Every restriction endonucleases will cleave the vector at its corresponding site except at the site Tetra which is not any specific site for these restriction endonucleases.