Total protein mg Activity Step Initial A salt precip 6540

Total protein (mg) | Activity Step Initial A salt precip 6540 | Specific activity | Yield (% units/mg) 4.6 10000 45644 35145 30142 76 B) Acid treat. 4455 C Ion exch.495 D) Phenyl Seph 135 E) affinity 6.8 43 17454 | 39.3 17550 15741 130.0 242.2 38 34 65 1) In this purification table, which step was most effective at purification? C) c A) A B) B D) D E) E 2) In this purification table, which step was the least efficient? A) A C) c D) D

Solution

Purification factor = specific activity step after /before (Measure of how effective the step is.)

ans 1 C

purification factor for ion exchange is high therefore it is most effective

ans 2 A

purification factor for salt exchange is least therefore it is least effective

step	specific activity	purification factor
initial	4.6
a	5	1.086956522
b	6.8	1.36
c	39.3	5.779411765
d	130	3.307888041
e	242.2	1.863076923