What specifically determines whether an enzyme is designated

What specifically determines whether an enzyme is designated a \"frequent cutting enzyme\" or a \"rare cutting enzyme\"? Include references. Based on this, are Nde1, EcoR1 and HincII frequent or rare cutters?

Solution

The recognition sequence of the restriction enzymes determine whether it is a rare cutter or frequent cutter.

Rare cutter are enzymes whose recognition sequence occur rarely in the genone

Frequent cutters\' recognition sequences are often observed in a genome.

The frequency of cutting is determined by the no of bases in recognition sequence = 4^n

Eg

EcoR1 - GAATTC - 6 bases

Frequency = 4^6 = 4096. Hence 1 in 4096 base pairs (6 bp length) are cleaved

Nde 1 - CATATG - 6 bases

Frequency = 4^6 = 4096. Hence 1 in 4096 base pairs (6 length) are cleaved

Hinc II - GTYRAC - 6 bases - similar to above examples

All the enzymes are frequent cutters.