Explain in 24 sentences why Type I and Type III restriction

Explain in 2-4 sentences why Type I and Type III restriction enzymes considered practical to use in research labs (review Chapter 4). Although there\'s no exact size cut-off, it\'s difficult to transform plasmids over 15 to 20 kilobases (kb) long (Review Chapter 4). What does this mean in terms of the potential double or super plasmid that your ligation tubes may contain? Do you think that it\'s likely that you will detect one of these double-plasmids? Explain in 2-4 sentences why or why not. What other method can be used to insert larger plasmids into cells? Method Name: In 2-4 sentences explain how this method works?

Solution

6.Type I restriction enzymes are complex multisubunit restriction and modification enzymes which cut the DNA at random sites far from their recognition sequence. Type III enzymes also cleave outside of their recognition sequence and need two such sequences in opposite orientations in the same DNA molecule for cleavage and seldom give complete digests.

7.a.The ligaton will occur successfully and the detection of the super plasmid will occur too.As ligation only depends upon the compatible sticky or blut ends and DNA ligase enzyme,the size of the plasmd won\'t matter in ligation.

b.1.Electroporation

2.Its a technique where electric field is applied to the cells to increase the permeability of the plasma membrane. This is used to insert foreign DNA or drugs into the cell.