What would most likely happen if you created a missense muta

What would most likely happen if you created a missense mutation in the phosphorylation site of a sensor kinase?

The phosphate group would be more efficiently transferred to the response regulator

Conjugation would not occur

Regulation through the 2-component system would be disrupted

There would be no effect

Solution

The two-component signaling system comprises a histidine kinase protein that receives a signal and transmits it, via phosphorelay, to a partner response regulator protein

In response to a signal, the histidine kinase auto phosphorylates a histidine residue in its carboxyl-terminal region and then transfers the phosphoryl group to an aspartate residue in the amino -terminal region of the partner response regulator protein.

Two-component systems regulate diverse responses including :
a) nutrient acquisition

b) energy metabolism

c) adaptation to physical or chemical aspects of the environment : chemotaxis, pH, osmolality, light quality

d) virulence : plasmid transfer (conjugation), degradative secretions, toxin production

Now if we created a missense mutation in the phosphorylation site of a sensor kinase then the auto phosphorylation event will not occur. As a result, the signal transduction process will be disrupted. Thus the regulations of diverse responses through 2-component system would be disrupted