RNA seq CCC ACG ACA GAA CAG UAU UUA AGG AGC UGC GAA GGU A A

RNA seq: CCC ACG ACA GAA CAG UAU UUA AGG AGC UGC GAA GGU

A. A. Sequence: Part B (0 points): Again, use Fig. 8.2 to fill in the 36-row table of RNA bases below & determine which mutational changes are synonymous (S) or non-synonymous (N)(10 points). To help you, I have filled in 3 of the 36 rows: RNA Base Nucleotide Position A C G U

Solution

RNA seq: CCC ACG ACA GAA CAG UAU UUA AGG AGC UGC GAA GGU

                RNA Nucleotide   Base position                        A             C             G             U

1.             C                                             1                              N             -               N             N            

2.             C                                             2                              N             -               N             N

3.             C                                             3                              S              -               S              S

4.             A                                             1                              -               N             N             N

5.             C                                             2                              N             -               N             N

6.             G                                             3                              S              S              -               S

7.             A                                             1                              -               N             N             N

8.             C                                             2                              N             -               N             N

9.             A                                             3                              -               S              S              S

10.          G                                             1                              N             N             -               N

11.          A                                             2                              -               N             N             N

12.          A                                             3                              -               N             S              N

13.          C                                             1                              N             -               N             N

14.          A                                             2                              -               N             N             N

15.          G                                             3                              S              N             -               N

16.          U                                             1                              N             N             N             -

17.          A                                             2                              -               N             N             N

18.          U                                             3                              N             S              N             -

19.          U                                             1                              N             N             N             -

20.          U                                             2                              N             N             N             -              

21.          A                                             3                              -               N             S              N

22.          A                                             1                              -               S              N             N

23.          G                                             2                              N             N             -               N

24.          G                                             3                              S              N             -               N

25.          A                                             1                              -               N             N             N

26.          G                                             2                              N             N             -               N

27.          C                                             3                              N             -               N             S

28.          U                                             1                              N             N             N             -

29.          G                                             2                              N             N             -               N

30.          C                                             3                              N             -               N             S

31.          G                                             1              N             N             -               N

32.          A                                             2              -               N             N             N

33.          A                                             3              -               N             S              N

34.          G                                             1              N             N             -               N

35.          G                                             2              N             N             -               N

36.          U                                             3              S              S              S              -