DNA shuffling can also be performed using multiple related g

DNA shuffling can also be performed using multiple related genes. Which of the following have been Improve in this manner. a. Insulin b. Erythropoietin c. Beta-lactamase d. Zinc fingers Which of the following is NOT an example of a combinatorial protein library. a. DNA coding for various protein domains recombined in a variety of ways. b. Exons and alternate exons being recombined in a variety of ways. c. Random PCR products from all over a genome are recombined into pieces of DNA and cloned. d. PCR products corresponding to protein motifs are randomly combined. The basis of many biomaterials is protein engineering with elastin-like polypeptides. Which of the following is NOT a result of this engineering. a. The ability of a doctor to inject liquids that then form gels. b. Zinc finger domains are sued to turn on elastin-like polypeptide genes. c. Peptides can promote cell migration and adherence. d. Movement and growth of vascular smooth muscle cells. Which of the following is NOT a domain associated with protein - protein binding a. Beta-sandwich b. Three-helix bundle c. Zinc finger motif d. Disulfide-bonded scaffolds e. Small scaffolds In order to produce recombinant proteins in mammalian cells, single cells must be Isolated. Match each of the technologies designed to isolate cell lines with its best description. Flow cytometry ______ Gel microdrop technology ______ Laser-enabled analysis and processing ______ b. Cells containing recombinant protein and reporter protein. The reporter is measured as the cells are sorted. c. Cells are immobilized, fluorescent antibody is added, the brightest cell is identified and the rest are killed. d. Lacz gene fusions and X-gal are used to measure activity. e. Cells containing recombinant protein are immobilized in a biotinylated agarose, the production of recombinant protein is captured and assessed by biotin-avidin reactions. Fluorescence is measured. With respect to HPLC, match each detector system below with its best description of that type. Refractive index ______ Ultraviolet ______ Fluorescence ______ a. Use of UV light to determine when a molecule passes through. b. Use light to determine when a molecule passes through and refracts light. c. Uses light to determine when molecules are absorbing light and emitting a different wavelength. d. Detects radioactively labeled molecules. Match the following each of the following proteases to its catalytic site protease class. Elastase _______ Papain _______

Solution

36. option C. Random PCR products from all over the genome are recombined into pieces of DNA and cloned.

Every gene is made of Exons and introns. Introns are removed from the gene during the splicing process. Due to the presence of introns, it will be impossible to get the protein when the plasmid is expressed.

37. b. Zinc finger domain is the DNA-binding domain of the transcriptional factor.

38 C. Zinc finger domain

Zinc finger domain is the DNA-binding domain of the transcriptional factor.

39 flow cytometry option B

gel micro drops technology option E

Laser-enabled analysis and processing - option C