SHow how to do 1 to 100 dilution for your 5uL Protien A samp

SHow how to do 1 to 100 dilution for your 5uL Protien A sample. (Diluent is H2O)

Hint: dilution factor(DF)=(Vinital + Vdiluent)/Vinital

Solution

dilution factor(DF)=(Vinital + Vdiluent)/Vinital

V initial+ V diluent = V final

and DF= 100

Vinitial =5uL

now, 100= V final/ 5

V final= 100*5=500uL.

ie., to make a 100x diluted protein sample, make it up to 500uL with water.[5uL protein + 495uL water]