Purpose 025 pt Unknown Data Table 45 pts Unknowns Wavelengt

Purpose (0.25 pt): Unknown # Data Table (4.5 pts) Unknowns Wavelength max (nm) Identity Absorbance Unknown Mixture Absorbance of Standard Concentration of Standard (ug mL-1) : 10 g mL-1 Value of k (mL g-1) Concentration of Unknown (ug mL.t) Accuracy of Unknown Concentration: (±0.5 g mL-1=[1.5 pts], ±0.51-1 g mL- 1 pt], > 1 g mL-1=[0.5 pt]) Dye 1 Dye 2 61Y3 lue/gren f6 1.3 477,7 ellow 0,230 10 g mL-1 FOR TA USE ONLY Purpose: O O

Solution

Ans. # Dye 1: Value of k = Abs of standard / Concertation of standard

                                                = 0.403 / (10 ug/ mL)

                                                = 0.0403 mL ug^-1

# Note that value of k is a constant for specified dye.

Now,

            k = Abs of unknown / Concertation of unknown

            Or, Concertation of unknown = Abs of unknown / k

                                                            = 0.230 / (0.0403 mL ug^-1)

                                                            = 5.7072 ug mL^-1

Therefore, [dye 1] in unknown mixture = 5.7072 ug mL^-1

# Dye 2: value of k = 1.321 / (10 ug mL^-1) = 0.1321 mL ug^-1

# [dye 2] in unknown mixture = 0.569 / (0.1321 mL ug^-1) = 4.3073 ug mL^-1