Which of the following is NOT based on nucleicacid hybridiza

Which of the following is NOT based on nucleic-acid hybridization? Which of the following is NOT based on nucleic-acid hybridization?

1.) DNA chip

2.) PCR

3.) Southern blotting

4.) Western blotting

5.) FISH

Solution

Western blotting is not a nucleic acid hybridisation. It is used to detect specific proteins by using the specific antibodies against the proteins. The target sample protein from the tissue or cell extract is electrophorated on the gel followed by incubating with specific antibodies against the target protein. Which can be detected by colorimetry, floroscence or radioactive depending on the type of the antibodies.