8 Problem The true absorbance of a certain sample is n but t
8 Problem: The true absorbance of a certain sample is\" n but there is 1.0% stray light that strikes the detector. Calculate the measured absorbance
Solution
Here
Atrue = log T-1 = 1
ot, T = 10-1 = 0.1
equation when stray light is used
Aobs = log [(1-T)S +T]-1
S = fraction of stray light = 1/100= 0.01, T = transmittance = 0.1
Aobs = log [(1-0.1)0.01 +0.1]-1 = or log [0.109]-1 = 0.9625
hence measured absorbance = 0.9625
