There are two sites of ADP phosphorylation in glycolysis The

There are two sites of ADP phosphorylation in glycolysis. These processes are called substrate-level phophorylations. Arsenate (AsO₄^-3) an analog of phosphate, uncouples ATP formation (interferes with) for the first ADP phosphorylation but not the second. Explain.

Solution

Arsenate is kinetically and thermodynamically unstable.There occurs instantaneous and spontaneous hydrolysis of arsenate.This is the reason why arsenate uncouples ATP formation (interferes with) for the first ADP phosphorylation but not the second.