Which of the following statements is not true about sequenci

Which of the following statements is not true about sequencing peptides with mass spectroscopy? a. The entire protein can be sequenced all at once using mass spectroscopy. b. Some purified proteins must be digested with proteases to eliminate undesirable characteristics such as hydrophobicity and solubility. c. Two rounds of mass spectroscopy are used to determine the sequence. d. In order to determine the sequence, a pure sample of protein is obtained through 2D-PAGE or HPLC. e. A database of protein ion spectra is used to compare the peaks of the unknown peptide to determine the sequence. Which of the following is used to quantify proteins with mass spectroscopy? a. 2H b. 33P c. 35S d. 32P e. 125| Why are protein tags useful? a. Protein tags are exactly the same thing as reporter fusions and perform similar functions. b. Tags allow the protein to be isolated and purified from other cellular proteins. c. Tags allow the protein to be quantitated. d. Protein tags enable the protein to which they are fused to perform their function more readily. e. None of the above.

Solution

Answer:

1) which of the following statement is not true about sequencing peptides with mass spectroscopy?

Answer: The entire protein can be sequenced all at once using mass spectrometry - Not True

2) Which of the following is used to quantify proteins with mass spectrometry?

Answer: a) ²H

3) Why are protein tags useful?

Answer: b) Tags allow the protein to be isolated and purified from other cellular proteins.