Which of the following is d palindrome restriction site 5CGH

Which of the following is d palindrome restriction site? 5\'CGHGAJ 5\' ACTACT 3 5\' CACGAG 3 5\' GCATGCT 5\' TGAAGT 3

Solution

The palindrome is a sequence of base pairs that reads same on the two strands when orientation of reading is kept the same.

The following sequence of GCATGC reads the same on the two strands in 5\' ----- 3\' direction.

This is also true when read in 3\' ---- 5\' direction. The orientation should not be changed.

Palindromic sequences play an important role in molecular biology. Restriction enzymes recognize these palindrome sequences and cut them in recombinant DNA technology methods.

Therefore, the sequence of 5’ GCATGC 3\' is a palindromic restriction site.