Procedures for the carbohydrate LAB Control experiment for m

Procedures for the \"carbohydrate LAB\" Control experiment for measuring glucose in an unknown solution 1. Get 10mL of distilled water in a 15ml conical tube and label it as coNTROL\" 2. Get 500HL of \"Unknown Glucose\" and pipette it into the CONTROL conical tube. 3. Take 50 uL of the \"Diluted unknown Glucose\" and pipette itin a centrifuge tube. r 4. Label the centrifuge tube \"Control\". 5. Add 950 uL of Glucose Affinity Reagent to the \"control centrifuge tube\" which contains the 50 HL of the \"Diluted Unknown Glucose\" already. r 6. Mix the solution in the \"control centrifuge tube\" manually for 10 secs. 7. Place the \"control centrifuge tube\" aside for 5 mins 8. Meanwhile set the spectrometer at 340 nm and blank it. 9. After 5 mins place the solution in a cuvette and place the cuvette in the spectrometer 10. Record the absorbance value at 340 nm. 33S A Measuring glucose in an milk 1. Get 10mL of distilled water in a 15mL conical tube and label it as \"DILUTED MILK.\" 2. Get 500 uL of \"Milk\" and pipette it into the \"DILUTED MILK\" conical tube. 3. Take 50 uL of the \"DILUTED MILK\" and pipette it in a centrifuge tube. 4. Label the centrifuge tube \"Milk\". 5. Add 950 HL of glucose Affinity Reagent o the Milk centrifuge tube which contains the 50 HL of the diluted \"DILUTED MILK\" already. 6. Mix the solution in the \"Milk centrifuge tube\" manually for 10 secs. 7. Place the \"Milk centrifuge tube\" aside for 5 mins 8. After 5 mins place the solution in a cuvette and place the cuvett ect rometer. 9. Record the absorbance value at 340 nm. Measuring lactose in an milk 1. Take 50 uL of the \"DILUTED MILK\" and pipette it in a centrifuge tube. 2. Label the centrifuge tube \"Milk Enzyme 3. Add 950 HL of \"REAGENT B-galactosidase\" to the \"Milk Enzyme centrifuge tube\" already. which contains the 50 uL of the diluted \"DILUTED MILK 4. Mix the solution in the \"Milk Enzyme centrifuge tube\" manually for 10 secs 5. Place the \"Milk Enzyme centrifuge tube\" aside for 5 mins 6. After 5 mins place the solution in a cuvette and place the cuvette in the Spectrometer 7. Record the absorbance value at 340 n

Solution

glucose concentration in milk

abs reasing at 340-> 5.0

measured by NADHafter dilution->2.8

In milk beforedilution=5*2.8=14

lactose concentration in milk:

abs reading at 340 nm=6.0

measured by NADH after dilution=2.0

inmilk before dilution=6*2=12