1 Explain how you might characterize the protein expression

1. Explain how you might characterize the protein expression of a cell line.

2. Name 5 reasons for scale-up of cell culture processes.

3. What is the first step before acquisition of sample tissue, regardless of species?

Solution

Answwer of question-

1-WE isolate the protien base on affinty tag or gfp or any tad then 2D analysis follow by Mass spectroscopy then data base analysis of protien to characterise the protien.

Question 2 answer- there many reason to scle up

i. Medium and nutrients.

ii. Oxygen.

iii. pH and buffer systems.

iv. Removal of waste products.

v- CO2

VI -cell condition

3 answer- Prepare media to keep sample and culture in suspention medium