Please help and why A was not correct A mixture of four prot

Please help and why? A was not correct.

A mixture of four proteins (A, B, C and E) is applied to a gel filtration column at pH = 7. Given the information supplied, which will elute first? A) A. B) B. C) C. D) A and E together. E) E.

Solution

When the protein mixture is applied to the column, B( with 105000 MW) would elute first because it is above the upper fractionation limit.