Production of transgenic animals requires transfections of Q

Production of transgenic animals requires transfections of

Question 11 options:

a stem cells

b eggs

c red blood cells

d All of the above

Solution

There are basically two methods in which embryonic stem cell were used to produce transgenic animal to be fertilized. Injection of Selected set of DNA using male pronucleus. This will interact with Egg and get fertilized. Genetic changes of erythrocytes required in case of trangenic animal production. Because RBC don\'t have protein translation system, Gene transfer require precursor like Lentiviral transduction, after that gene transcribed in red blood cell.

So option d is correct one