A PCR reaction begins with 4 double stranded segment of DNA

A PCR reaction begins with 4 double stranded segment of DNA. Part A Estimate the number of double-stranded copies of DNA that are present after the completion of 15 amplification cycles?

Solution

The number of double stranded copies originating from one template DNA is given by 2⁽ⁿ⁺¹⁾, where \'n\' is the number of cycle.

thus from one template DNA, at the end of the 15th cycle, we will have, 2⁽¹⁵⁺¹⁾= 65,536.

if we have 4 template DNA, then the result will be 4*65,536 =262,144.