why is it advisable to perform this test on a known catalase

why is it advisable to perform this test on a known catalase-positive organism along with the organism you are testing?

Solution

Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also, some cells use catalase to destroy cellular debris or worn out organelles. In this lab, we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme.

.In general, enzymes are proteins produced by living cells; they act as catalysts in biochemical reactions. ..A catalyst affects the nte of a chemical reaction. ..One consequence of enzyme activity is that cells can cany out complex chemical activities at relatively low temperatures.

.....In an enzyme-catalyzed reaction, the substance to be acted upon (the substrate = S) binds reversibly to the active site of the enzyme (E). ..One result of this temporary union is a reduction in the energy required to activate the reaction of the substrate molecule so that the products (P) of the reaction are formed.

.....In summary:

E+S -> ES -> E+P

.....Note that the enzyme is not changed in the reaction and can be recycled to break down additional substrate molecules. ..Each enzyme is specific for a particular feaction because its amino acid sequence is unique and causes it to have a unique three-dimensional structure. ..The active site is the portion of the enzyme that interacts with the substrate. so that any substance that blocks or changes the shape of the active site affects the activity of the enzyme