Under ideal PCR condition Starting with a single copy of the
Under ideal PCR condition, Starting with a single copy of the target gene, calculate the number of gene copies in solution after 30 cycles of replication?
Please explain in detail and let me know if the equation 2^(cycle # +1) would work. thanks
Solution
for example you start with 2 templates (1 piece of dsDNA). How many pieced do you have after 1 cycle? (4).
And after a second cycle ? (8) and a third? (16) ... you a pattern here?
Nn=N0X.2n with N0is your start template (how many pieced you have for the primers to attach to), Nnis the number copies of DNA you have, with small n the #of cycles.
Nn= 2* 230