A small oligonucleotide has the sequence 5 dAdCdT3 i What is

A small oligonucleotide has the sequence 5\' dAdCdT-3. i) What is the sequence of the deoxyribose oligo nucleotide that is complimentary to this small sequence? ii) What is the sequence of the ribo oligonucleotide that is complementary to the sequence you gave in i? iii) Draw the complete structure of the ribo oligonucleotide you gave as answer to ii. Make this as correct as possible and be sure to show the correct connecting bonds and the correct ends You have the following mRNA sequence: A G A U G A A A G A C G C G C U A U U G U G A C G U Using the codon table provided for you and keeping in mind start and stop codons, what would be the sequence of amino acids in the peptide that would be transcribed from this mRNA sequence? The following is a polypeptide with the sequence given. On this sequence underline the amino acids region that would be most likely form a transmembrane alpha helix in this sequence. You should circle a 19-20 amino acid long segment. You may underline the region or give the region by the numbers of amino acids (such as 10-30 or something like that). ii The N-terminus of transmembrane proteins of plasma membranes is found on the exterior of the cell and the C- terminus usually found in the cytosolic of the plasma membrane. Knowing that, also circle the individual amino is acids that could have carbohydrates attached to them in this transmembrane glycoprotein.

Solution

1. 5 \' dAdCdT 3\'

i) 3\' dTdGdA 5\' ; deoxy oligo nucleotide sequence

ii) 5\' dAdCdU 3\' complementary ribo oligonucleotide sequence

2. Reading frame 1-

R Stop R R A I C D

R- arg; A- ala; I - his; C- cys; D aspartate

Arg- stop- arg-arg-ala-his-cys-asp

3. I) alpha helix can be formed between 8-29; this is because gly and pro residues do not have helix forming propensities, thus, the area excluding the same would be included in the same.

ii)