How much of the 18 M TAE buffer would you need to use if you

How much of the 1.8 M TAE buffer would you need to use if you were making 25 ml of 100 mM TAE ? Answer should be in ml. Be precise and use a calculator.

Solution

Please find the answer below:

Molarity of stock solution: 1.8 M or 1.8 * 10⁶ mM

Molarity required in the dilute solution: 100 mM

volume required of the dilute solution: 25 ml

Let us assume that the volume of stock solution required is V ml.

Thus, according to the molar equation:

M1V1 = M2V2

here, putting the values:

1.8 * 10⁶ M * V = 100mM * 25

This gives, V = (100 * 25) / 1.8 * 10⁶ ml of stock solution

This gives, V = 25000 / 10⁶ ml of solution

or V = 0.25 ml

Thus, the stock solution should be taken 250 ul and diluted to 250 ml with water to get a resultant dilute soution of 100 mM concentration.