2 Under which of the following conditions do you expect to s

2. Under which of the following conditions do you expect to see treadmilling of the actin filament? The critical concentrations of F-actin are: (+)-end: 0.12 microM (-)-end: 0.6 microM (1 point)

a. The cytosolic concentration of G-actin-ATP is 0.4 microM, but you have added 0.3 microM of the sequestering protein thymosin to your reaction. Thymosin binds to G-actin ATP in a ratio of 1:1.

b. The cytosolic concentration of G-actin-ATP is 0.15 microM and of G-actin ADP is 0.2 microM.

c. The cytosolic concentration of G-actin-ATP is 0.7 microM, but your reaction also contains profilin, an enzyme that converts G-actin-ADP into G-actin-ATP.

d. The cytosolic concentration of G-actin-ATP is 0.4 microM, but you have also added the (-)-end capping protein tropomodulin to your reaction

e. The cytosolic concentration of G-actin-ATP is 0.1 microM

Solution

Treadmilling of the actin filament is senn when e. The cytosolic concentration of G-actin-ATP is 0.1 microM