A series of cell culture samples each expected to have diffe

A series of cell culture samples, each expected to have different Ca^+2 expression levels due to their different states of hypoxia, were analyzed using two different techniques; fluorescence ativated cell sorting (FACS) and fluorescence resonance energy transfer (FRET). The results of the Ca^+2 expression levels from the FACS analyses provided values of

Sample 1= 3.2 mM

Sample 2= 12.8 mM

Sample 3= 7.3 mM

Sample 4= 1.0 mM

Sample 5= 5.4 mM

Sample 6= 6.1 mM

Sample 7= 2.2 mM

The results from the FRET analyses for the exact same 7 samples revealed values of;

Sample 1= 2.8 mM

Sample 2= 12.6 mM

Sample 3= 7.7 mM

Sample 4= 1.2 mM

Sample 5= 5.5 mM

Sample 6= 5.6 mM

Sample 7= 2.7 mM

Based on these results , is the accuracy of the FRET analysis method the same as that of the FACS analysis method at the 95% confidence level? (Show calculation)

Solution

Ho : Mean (FACS) = Mean (FRET)

Ha : Both the means are different

We carry out t-stat test. The calculation is shown in the tabular format

Since the two tailed critical value (2.4469) is greater than calculated t-stat ( -0.0971), the null hypothesis can\'t be rejected.

Thus, we can say that the two tests give the same results.

Hope this helps. Ask if you don\'t understand anything.