You need to make MM histidine leucine plates How much histid

You need to make MM +histidine +leucine plates. How much histidine should you add to the minimal media plate if your stock solution is 1500X? The volume of the plate is 25 ml. If you are having trouble with this question, try some practice problems in Yeast as a model system. 60 µl 16.7 µl 0.06 µl Cannot determine from the information given 0.0167 µl

Solution

Answer:

Stock of ethanol = 1500x

Required stock = 1x

Volume = 25 mL

C1V1= C2V2

(stock)   (required)

V1=C2V2/C1

V1 = (1x X 25 mL )/ 1500x

=0.016 mL

=16.7 µl