8 A cytologist needs to test if a virus replicates in the cy

8. A cytologist needs to test if a virus replicates in the cytoplasm or the nucleus of the live host cells. The cytologist should use a _______ for the examination.

A. A SEM

B. A TEM

C. A darkfield LM

D. A high-end STED LM

E. A confocal microscope

Solution

Answer: (D). A high-end STED LM

STED LM or stimulated emission depletion light microscopy is used to visualize the replication process of virus in live host cells. It is a newly invented light imaging technique with super lateral resolution and aids in observing sub-cellular compartments which are not possible with other microscopy techniques.

Hence a cytologist can use high-end STED LM to observe whether viruses are replicating in the cytoplasm or nucleus of host cell.