Absorption Fluorescence emission Absorption uorssiu 400 450

Absorption Fluorescence emission Absorption uorssiu 400 450 500 550 600 650 700 750 550 wavelength (nm) wavelength (nm)

Solution

The dual band fluorescence filter portfolio includes three carefully balanced combinations that contain dual bandpass excitation and emission filters, which are incorporated into a single element capable of selectively isolating fluorescence emission from two fluorophores simultaneously.They  they are equally effective with alternate pairs of fluorescent probes that have similar absorption and emission spectral profiles.

Performance of the dual band filter sets can be judged by comparing images from the same viewfield captured with each of the individual filter combinations. The specimen is an adherent culture of Indian Muntjac deer skin fibroblast cells that were fixed and stained with a combination of three fluorophores. The nuclei were stained with the nucleic acid specific fluorophore DAPI (violet excitation and blue emission), while the filamentous actin cytoskeletal network was labeled with Alexa Fluor 488 (similar spectral characteristics to fluorescein; blue excitation and green emission) conjugated to phalloidin. Before fixation, the cells were incubated with MitoTracker Red CMXRos targeted at the actively respiring mitochondria. The absorption and emission spectral profiles of MitoTracker Red CMXRos (green excitation and orange-red emission) are centrally positioned in wavelength range between TRITC and Texas Red.