Below is a sequence of double stranded DNA that includes onl

Below is a sequence of double stranded DNA that includes only the beginning of gene X. The underlined sequence represents the promoter and transcription start is indicated with a +1. The start codon is boxed. Label the template and RNA like strands. Label the polarity of each strand Write the first ten nucleotides of the mRNA.

Solution

                                                                                                                                 +1

A. Given DNA sequences : 5\' TGGGCTCATCTGATCCATCCGGTCCGGTCCCGTCCAC 3\'

                                             3\' ACCCGAGTAGACTGGTAGGCCAGGCCAGGGCAGGTG 5\'

Label template strand and RNA like strands: bottom sequence 3\' to 5\'

B. polarity of each strand : 5\' TGGGCTCATCGATCCATCCGGTCCGGTCCCGTCCAC 3\'

                                          3\' ACCCGAGTAGCTAGGTAGGCCAGGCCAGGGCAGGTG 5\'

C. First ten nucleotides of mRNA : 5\' to 3\' end of RNA

                                                 5\' CACCUCCCUGGCCUGGCCUACCUACUCGGGU 3\'