What volumes of sample aliquot and diluent would you require

What volumes of sample aliquot and diluent would you require to prepare a 1:1000 dilution in a final volume of 10 mL?

Solution

Consider the formula, C1V1 = C2V2.

Where, C1 = Concentration of the stock = 1

V1 = Volume of stock to be taken = ?

C2 = Concentration of final volume = 1/1000

V2 = Volume of final solution = 10 mL

Now, substitute the values in the given formula.

1* VI = 1/1000* 10

V1 = 0.001 mL

Thus, take a volume of 0.001 mL of aliquot and makeup the volume up to 10 ml. The resulting solution\'s concentration is 1:1000.