can methylene blue stain simple stain be used to determine i

can methylene blue stain (simple stain) be used to determine if bacteria is anaerobic or aerobic?
is there a another dye that may confirm this?
thanks you.

Solution

Yes, methylene blue which is a simple stain can be used to determine whether the bacteria in our culture medium is aerobic or anaerobic.

Resazuin is also a redox indicator that can be used in the laboratory apart from methylene blue.

Advantages of using methylene blue:

1. It is cheap.

2. Easily accessible dye.

3. Widely used in microbiological laboratories

4. Results obtained with this dye will be more stable over time.