product Pormahan In order to make the reaction occur the mix

product Pormahan In order to make the reaction occur, the mixture must contain both substrate and enzyme. Name the specific components for this reaction: Substrate aoto sidase la chse Based on the information given in Table 1, you will set up four sample tubes to illustrate how this method works. Table 1. Reaction Mixtures for Lactose Experiment L break lactose PBS buffer 10 mM ONPG (mL) 3.7 Blank 4.7 Answer the following questions. What hypothesis is being tested? What is the independent variable in this experiment?

Solution

Answer:

(Since only the table has been provided and nothing have been mentioned about the aim of the experiment, it would be tough to give a specific answer to the questions. The questions appear to be continuing parts of a previous problem)

The parameters from the table provided gives an idea about a lactase catalyzed reaction with PBS as the buffer and the colorimetric substrate, ONPG.

1. The hypothesis being tested:

Hypothesis being tested is generally refers to the question we are trying to address. For example, we can test for a hypotheisis such as the effect of a competing substrate on the activity of lactase.

2. The independent variable is the parameter you are manipulating or testing. The independent variable is plotted on the horizontal axis (x-axis).

The dependent variable is the parameter you are measuring. The dependent variable is plotted on the vertical axis (y-axis).

Both the parameters will dependent on the question you are trying to address.