What complonents of PCR use chemical methods Which use physi

What complonents of PCR use chemical methods? Which use physical methods?

Solution

Polymerase chain reaction (PCR) technique used to amplify a given sequence of DNA by several folds.

PCR machine is a thermocycles(helps in increasing and decreasing temperature)

PCR mix is a mix of chemicals such as DNA, dinucleotides, primers, Polymerase enzymes.

The double stranded DNA is separated by heating to 95C. And cooling to 55C to anneal the primers (short complementary sequences). Separation and annealing use physical method.

The chemical reactions:

Primer annealing and polymerization by the enzyme Taq DNA polymerization using dinucleotides is an enzyme driven chemical process.