Explain Hayflick and Mooreheads experimental design to show

Explain Hayflick and Moorehead\'s experimental design to show that cells in culture (untransformed) have a limit to how many times they can be passaged.  Please 1 paragraph and straight forward.

Solution

Hayflick and moorhead suggested that duw to telomere shortening every sucessive cell division the cells eventually stopdividing and die and there is a limit to number of cell divisions it can go probably around 40-45

Experimantal setup

Hayflick and Moorhead human origin normal male fibroblasts that had divided about 40 times with female fibroblasts divided only about 10 times . Unmixed cells were used as experimental controls. As male control cells stopped dividing what they found was mixed culture contained only female cells. This implied that old male control culture cells remembered they were divided already about 40 times, even if they were mixed with young cells, they also found technical experimental errors and virus contamination were improbable explanations as they reasoned why only male culture cells had died. They found cells stopped dividing based upon how many cell divisions the cells earlier had. ...

Hope it answers your question ... should u need further clarification feel free to comment .