An initial enzyme n the oxidation of palmitic acid is palmil

An initial enzyme n the oxidation of palmitic acid is palmiltoyl-coA dehydrogene (PCBH) ypu have isolated a form of the enzyme from rat liver and performed enzyme steady-state kinetic analysis using palmitoyl-CoA as a substate where the [PDCH]=0 12, the following data were obtained Plot velocity vs. [palmitoyl -CoA]and estimate v_max and k Plot 1/velocity vs 1/[palmitoyl CoA]and Determine v_max and k_m. Determine K_cat for this sustrate Catalytic antibodies are prepared a transition state analog of a substrate what is a transition state analog? Why do these antibodies erzymatic activity? Do catalytic antibodies work because they maximize k_cat or minimize k_m? An enzyme has a single active site at when it can bind and hydrolyze either X or Y. The enzyme cannot bind X and Y at the same time will k_m for x be affected if Y is present in the reaction mixture? Explain. will v_Max for x affected if Y is present in the reaction mixture? Explain. why are inhibitors necessary as enzymatic control mechanisms in biological systems? How do the different types enzymes inhibition effect K_m and v_max ? why is regulatory enzyme often the first enzyme in a multiple reaction sequence? How are allostene enzymes different from other enzymes?

Solution

5. Regulatory enzymes are commonly the first enzyme in the multienzyme system. since the product of the reaction catalysed by first enzyme acts as a substrate of the second enzyme so the cell can control the amount of resulting product by regulating the activity of first enzyme.

Regulatory enzymes exhibit increased or decreased cataltic activity in response to various signals available. They can increase the catalytical activity or they can decrease the catalytic activity.

eg. Allosteric enzyme

Allosteric enzymes function through reversible, noncovalent binding of a regulatory metabolite called a modulator.

4. Inhibitors are needed as enzymatic control mechanism since there should be some inhibitor present in the cell to maintain its concentration. Feed back regulation take place where the product formed itself act as a inhibitor to stop the enzymatic acivity so that the concentration of the product does not increases in the cell which can lead to disease production