Describe what would be seen under a brightfield vs darkfield

Describe what would be seen under a brightfield vs darkfield microscope viewing a thin section of pear as the specimen. What are some of the characteristics and descriptions of the pear.

Solution

Difference between dark field and brightfield microscope

Bright field microscopy (BM) is a conventional technique and suitable for viewing natural colors of a specimen or a stained samples.Specimen observes darker on a bright background, Here the pear appears dark against a bright background..It shows a cross section of the vascular tissue ina plant stem.

Darkfile shows the pear against a dark background. Specimens viewed under dark field microscopy are aquatic orgnisms like algae, plankton, livebacgeria. The pear has to be stained before placing under the dark field microscopy. We can obtain high contast, high resolution images if viewed under dark field microscopy.