You must cleave the following peptide into smaller fragments

You must cleave the following peptide into smaller fragments. Larynmtqgrckpvntfvmhepl Vdvqnvcfkeaaak expect to find: E) How many different possible arrangements of disulfide bonds are possible in this peptide? F) Which exopeptidase could you use to determine the C-terminal amino acid? Cleavage of a short, unknown peptide with trypsin yields the following three peptides: Leu-glu; gly-tyr-asn-arg; and gln-ala-phe-val-lys Cleavage of the peptide with chymotrypsin yields the following three peptides: Gln-ala-phe; asn-arg-leu-glu; val-lys-gly-tyr What is the sequence of the peptide? You are trying to determine the sequence of an unknown inflammatory protein, by fragmentation analysis. Unfortunately, you have very little starting material, so you can only sequence 4-5 residue of each peptide before you run out. Although your protein is a single peak by chromatography, the sequencing is ambiguous. You react it with mercaptoethanol and iodoacetate, and find that you have TWO peptide chains, call them \"A\" and \"B\". So you purify them separately by chromatography, and obtain the following results. Peptide \"A\" Amino acid analysis: C*:1, D:1, E:2, I:2, M:2, R:1, S:1, T:4 (C* means S-carboxymethylcysteine, the product of cysteine after the above treatment) (Question: you see a \"Q\" in the sequencing, but not the AAA. Why?) After cleavage with trypsin, you purify two peptides.

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