A sequence of DNA was analyzed with a bioinformatics program

A sequence of DNA was analyzed with a bioinformatics program that translated the sequence in all 6 possible reading frames. Below is the outcome of this analysis. If this sequence contains one gene, which frame is most likely the correct reading frame for this gene? (Remember that Met represents a potential start codon and Stop represents a stop codon.) 5\'-3\' direction, Frame 1 AL K Stop G Met G G H S V G P S C W R P R, D L S L A S R S H W S L A A G T L W S Stop P A P H L Stop T V P Q G P A S G S R Met V W G W C P Q T A S W W G L G G C R Y S Met P P Met R Met L G P T A A G S G S P S A S C A I S A 5\'-3\' direction, Frame 2 R Stop N E G W G G I Q W A P P A G D P G T Stop A W P A G A T G L W Q R G H C G A D L P L T Y R Q S H R A Q H L G Q G W C G A G A L R P H P G G A S A A A G S T Q C L P Stop G C W D L Q L P A A A H P A H P V P F Q R 5\'-3\' direction, Frame 3 A E Met R D G G A F S G P L L L E T P G P E P G Q Q E P L V F G S G D T V E L T C P S P I D S P T G P S I W V K D G V G L V P S D R I L V G P R R L Q V L N A S H E D A G T Y S C R Q R L T Q R I L C H F S V Stop 3\'-5\' direction, Frame 1 H A E Met A Q D A L G E P L P A A V G P S I L Met G G I E Y L Q P P R P H Q D A V Stop G H Q P H T I L P D A G P C G T V Y R Stop G A G Q L H S V P A A K D Q W L L L A R L R S R G L Q Q E G P T E C P P I P H F S 3\'-5\' direction, Frame 2 T L K W H R. Met R W V S R C R Q L Stop V P A S S W E A L S T C S R R G P T R Met R S E G T S P T P S L T Q Stop L G P V G L S I G E G Q V S S T V S P L P K T S G S C W P G S G P G V S S R R G P L N A P P S L I S A 3\'-5\' direction, Frame 3 R Stop N G T G C A G Stop A A A G S C R S Q H P H G R H Stop V P A A A E A P P G C G L R A P A P H H P Stop P R C W A L W D C L Stop V R G R S A P Q C P R C Q R P V A P A. G Q A Q V P G S P A G G A H Stop P P H P S F Q R

Solution

Look the 5\'-3\' frame 1 , 2 which has more than one stop codon even in middle of sequences alsomthey both start with stop codon. same we see in 3\'-5\' direction frame 1, 2 and 3 all of them are having more than one stop codon with met sequecne any where in between .

The most correct frame sequence is 5\'-3 direction Frame 3 which start with Met and only one stop codon at the end of sequences.