Preparing a serial dilution Serial Dilution Volume 100 ml In

Preparing a serial dilution: Serial Dilution Volume: 100 ml Initial Concentration: 10% glucose (weigh 10g glucose, add distilled water to bring the total volume to 100 ml.) Molecular weight of glucose C_6H_12O_6 = 180.1559 g/mole Molarity = mole of solute/hitters of solution What is the molarity of a 10% glucose solution^2(10 g glucose in 100ml solution) Convert grams to moles: 10g glucose x 1 mole/180.1559 g = 0.0555 moles glucose Divide moles by liters of solution: 0.0555 moles glucose/0.1 L solution = 0.555 M (0.555 moles per liter) Calculate the concentration and recipe for a serial dilution beginning with a 0.555M glucose solution. Preparing 100ml of a 5% glucose solution (for the effect of temperature experiment): Recipe: Molarity:

Solution

10% glucose

10 teams of glucose diluted with water to make 100 ml.

Concentration - 0.555M

1% glucose

1 gram of glucose diluted with water to make 100 ml

Concentration - 0.055M

0.1% glucose

0.1 gram of glucose diluted with water to make 100 ml

Concentration 0.00555M

0.01%

0.01 grams of glucose diluted with water to make 100 ml

Concentration - 0.000555M

100 ml of 5% glucose

5 grams of glucose dissolved in water to make 100 ml

Concentration 0.2775 M