Consider the mutant hemoglobins with the given amino acid su

Consider the mutant hemoglobins with the given amino acid substitutions: Hb D (alpha 68) lys instead of asn Hb J (beta 569) asp instead of gly. Hb N ((beta 95) glu instead of lys Hb C ((beta 6) lys instead of glu. On electrophoresis at pH 8.6, the order in which the bands will be located (from anode to cathode) is: A. C, J, D, N B. N, J, D, C C. J, N, D, C D. N, D, J, C E. D, C, N, J

Solution

Answer: (B)

Explanation:

1. HbD: Lys (positive charge) instead of Asn (no charge).

2. HbJ: Asp (negative charge) instead of Gly (no charge)

3. HbN: Glu (negative charge) instead of Lys (positive charge)

4. HbC: Lys (positive charge) instead of Glu (negative charge)

The net negative charge determines the migration rate of the protein samples in gel. The largest change towards negative charge is for HbN while the lowest is for HbC.