1 youve just spent 200 to prpduce 1000U on an enzyme from si
1) you\'ve just spent $200 to prpduce 1000U on an enzyme from sigma. the specific activity of the enzyme is listed as 100U/mg. how many mg of protein should you have in the bottle?
2)you\'ve just spent $200 to prpduce 1000U on an enzyme from sigma. the specific activity of the enzyme is listed as 100U/mg. you need 25U to carry out an experiment. how many mg of the protein will you need for your experiment.
Solution
(2)
Answer:
Stock: 100U/mg
Required: 25U
100U---->1mg
25U----> 25/100=0.25 mg
you need f0.25mg of the protein for your experiment.
