Homework Sourseidentify the components of the following media DNAase plate
Solution
DNase Test Agar Base :DNase Test Agar Base is for detection of deoxyribonuclease activity of and for identification of pathogenic Staphylococci.
Composition**
Ingredients Gms / Litre
Casein enzymic hydrolysate 15.000
Papaic digest of soyabean meal 5.000
Deoxyribonucleic acid (DNA) 2.000
Sodium chloride 5.000
Agar 15.000
Final pH ( at 25°C) 7.3±0.2
Columbia C.N.A. Agar Base is used for selective isolation of pathogenic gram-positive cocci
Composition**
Ingredients Gms / Litre
Biopeptone 20.000
Tryptose B # 3.000
Corn starch 1.000
Sodium chloride 5.000
Colistin sulphate 0.010
Nalidixic acid 0.015
Agar 15.000
Final pH ( at 25°C) 7.3±0.2
Mannitol Salt Agar is used as a selective media for the isolation of pathogenic Staphylococci.
Composition** Ingredients Gms / Litre
Proteose peptone 10.000
Meat extract B# 1.000
Sodium chloride 75.000
D-Mannitol 10.000
Phenol red 0.025
Agar 15.000
Final pH ( at 25°C) 7.4±0.2
Phenylethyl Alcohol Agar is a selective medium for the isolation of gram- positive organisms like Staphylococci and Streptococci.
Composition** Ingredients Gms / Litre
Casein enzymic hydrolysate 15.000
Soya peptone 5.000
Sodium chloride 5.000
Phenylethyl alcohol 2.500
Agar 15.000
Final pH ( at 25°C) 7.3±0.2
BD Trypticase Soy Agar II with 5% Sheep Blood Formula* Per Liter Purified Water
Pancreatic Digest of Casein 14.5 g
Papaic Digest of Soybean Meal 5.0
Sodium Chloride 5.0
Growth Factors 1.5
Agar 14.0
Defibrinated Sheep Blood 5% pH 7.3 +/- 0.2
Bile Esculin Agar Base is a differential medium for isolation and presumptive identification of group D Streptococci
Composition** Ingredients Gms / Litre
Peptone 5.000
Meat extract B # 3.000
Bile 40.000
Ferric citrate 0.500
Agar 15.000
Final pH ( at 25°C) 6.6±0.2
Chocolate agar Base is recommended for the isolation of Neisseria gonorrhoeae
Composition** Ingredients Gms / Litre
Proteose peptone 20.000
Dextrose 0.500
Sodium chloride 5.000
Disodium phosphate 5.000
Agar 15.000
Final pH ( at 25°C) 7.3±0.2
Thayer Martin Agar Plate For selective isolation of Gonococci from pathological specimens.
Composition** Ingredients Gms / Litre
Peptone, special 23.000
Starch 1.000
Sodium chloride 5.000
Agar 13.000
Cystine Tryptone Agar is recommended for maintenance, subculturing, detection of motility
Composition** Ingredients Gms / Litre
Casein enzymic hydrolysate 20.000
L-Cystine 0.500
Sodium chloride 5.000
Sodium sulphite 0.500
Phenol red 0.017
Agar 2.500
Final pH ( at 25°C) 7.3±0.2
MARTIN LEWIS AGAR WITH LINCOMYCIN,
NYC Agar Base is recommended for the selective isolation of gonococci .
Composition** Ingredients Gms / Litre
Proteose peptone 15.000
Corn starch 1.000
Glucose 5.000
Sodium chloride 5.000
Dipotassium hydrogen phosphate 4.000
Potassium dihydrogen phosphate 1.000
Agar 20.000
Final pH ( at 25°C) 7.4±0.2
| Hemoglobin, Bovine | 10.0gm |
| Pancreatic Digest of Casein | 7.5gm |
| Selected Meat Peptone | 7.5gm |
| Sodium Chloride | 5.0gm |
| Dipotassium Phosphate | 4.0gm |
| Monopotassium Phosphate | 1.0gm |
| Corn Starch | 1.0gm |
| Colistin | 7.5mg |
| Trimethoprim Lactate | 5.0mg |
| Amphotericin B | 1.5mg |
| Vancomycin | 1.0mg |
| Lincomycin | 1.0mg |
| Koenzyme Enrichments | 10.0ml |
| Agar | 10.0gm |
