What is the temperature requited to denature myoglobin witho

What is the temperature requited to denature myoglobin without guanidine?What is delta S and delta H of myoglobin denaturation without guanidine?

Solution

he temperature to denature myoglobin without guanidine is 30 degree C(celsius).

Go= Ho TSo = RT ln KD     ->(1)

Ln KD =(HoTSo)/RT    ->2

lnKD=Ho/RT+ So/R( 3)

dlnKD/ d(1/T)=Ho/R=H0vHoff/R(4)

or

dlnKD/dT=H0vHoff/RT2(5)