An arg cys his met pro bacterial strain is known to be lysog

An arg^+ cys^+ his^+ met^+ pro^+ bacterial strain is known to be lysogenic for a newly discovered phage that undergoes specialized transduction. The bacterial chromosome map is... The lysogenic strain is used as a source of the phage, and the phages are added to a bacterial strain of genotype arg^+ cys^+ his^+ met^+ pro^+. After a brief incubation, samples of these phage-exposed bacteria are placed on five different types of minimal media (1-5), with the supplementations as indicated in the following table. A plus sign means the minimal media contained that particular supplement (indicated at the top of each column), a minus sign means the media was missing that supplement. Based on this information, tell me whether or not you\'d find colonies (growth) on each of the five media if specialized transduction occurred. OK cool. Why would those media have grown colonies? (lessthanorequalto 3 sentences) you perform a generalized transduction experiment, where phages are collected from an S. aureus donor strain of genotype abc+ def+ ghi+, then used to transduce a recipient of genotype abc- def- ghi-. The recipients were plated on minimal medium supplemented with def and ghi. you see lots of colonies. List the genotypes found in the colonies Colonies are replicated onto three different media, (1) minimal plus ghee only, (2) minimal plus def only, (3) minimal. in theory, what genotypes would grow? Growth was observed at 58% on medium 1, 7% growth was observed on medium 2, and no colonies observed on medium 3. What are the genotypes of the colonies on each medium?

Solution

Answer:

2. a. Media lacking Arg and His are the two media where there is a possibility of finding colonies.

Media lacking Met, Cys and Pro should not have any growth.

b. This is because, Arg and His are near to the attachment site, and there is a very high probability of these two genes getting transferred during the specialized transduction process.

Only those genes near the integration site are possible candidates for misincorporation into phage particles that then deliver this DNA to recipient bacteria.

Media lacking Met, Cys and Pro should not have any growth since they are far away from the attachment site.