Homework Soursewhat Is the purpose of heating the slide during the staining
what Is the purpose of heating the slide during the staining process ?
A.) allows for further penetration of the stain through the lipoidal wall into the cytoplasm
B.) eliminates contaminating bacteria
C.) kills the bacteria and adheres it to the slide
what Is the purpose of heating the slide during the staining process ?
A.) allows for further penetration of the stain through the lipoidal wall into the cytoplasm
B.) eliminates contaminating bacteria
C.) kills the bacteria and adheres it to the slide
what Is the purpose of heating the slide during the staining process ?
A.) allows for further penetration of the stain through the lipoidal wall into the cytoplasm
B.) eliminates contaminating bacteria
C.) kills the bacteria and adheres it to the slide
Solution
You flame the loop before to destroy any organisms already on it to prevent contamination of your culture You flame it afterward to prevent unintended spread of bacteria especially if the bacteria you are culturing is highly virulent Heat fixation allows the bacteria to stick to the slide,instead of rinsing off with the various rinses used during the staining process Heat fixation is the procedure used to adhere a bacterial smear to a glass slide for viewing by a compound microscope.It denatures the proteins on the surface of the bacteria causing them to be sticky After a smear has been allowed to dry at room temperature,the slide is gipped by tungs or a clothspin and passed through the flame of a bunsen burner several times to heat-kill and adhere the organism to he slide Heat fixation cannot be used in capsular stain method as heat fixation will shrink or destroy the capsule and cannot be seen in stains Sterilizing of inoculation loop is essential in order to avoid contamination of slide preparations and also to prevent contamination of inoculum plate
