A variety of media and culture conditions can be used to cul

A variety of media and culture conditions can be used to culture and identify Listeria monocytogenes. For each, characterize it as selective or differential.

Solution

Listeria monocytogenes is basically a soil-borne micro-organism. It has been found in various sources such as water, agricultural products, and in animals. Listeria monocytogenes has been known as etiologic agent of listeriosis, causing meningitis, encephalitis, septicemia and endocarditis in humans.

various selective and differential methods were developed to isolate the Listeria monocytogenes species as follows

Selective

Incubation at 30 degree celsius

Adding antibiotics such as nalidixic acid

Adding high concentarion of salts such as lithium chloride and sodium chloride

Adding antifungals such as amphotericin B

Differential

Adding pH indicator to detect ferementaion of rhamanose to acid

Detecting beta heamolysis