Which of the following is not a general feature of DNA repli
Which of the following is not a general feature of DNA replication?
____________ studies provide important information about the enzymes involved in DNA replication.
E. coli chromosomal replication begins at _________.
The bacterial core polymerase has two functions: 5\'3\' polymerase activity and _________________ activity.
Bacterial DNA polymerase III holoenzyme catalyzes ____________________ strand synthesis.
| semiconservative |
| semidiscontinuous |
| bidirectional |
| conservative |
| none of the above |
Solution
1. DNA replication is a process where one new strand is formed from the parental template strands. it is semi-conservative and consists of two replication forks (Bidirectional ) i.e. leading strand (Continuous DNA replication) and lagging strand or semidiscontinuous strand (Okazaki fragments).
Therefore, the correct option is: conservative
2. Mutation studies play an important role to study the effect of a specific mutation on the production of a particular enzyme or protein. Therefore, in order to study the enzymes involved during DNA replication, various mutations can be carried out.
Hence, the correct option is:Mutant studies
3. In E.coli, the replication starts at a single origin site (Monocistronic) known as origin of replication or oriC. This site has 3 Adenine–Thymine rich repeats.
Therefore, the correct option is: oriC
4. Bacterial core polymerase has two functions: 5......>3\' polymerase activity and 3’ 5’ exonuclease activity (proof reading).
Therefore, the correct option is 3\'5\' exonuclease
5. Bacterial DNA polymerase III has 5’ 3’ direction polymerase activity and lack 5’ 3’ exonuclease activity. This is capable of polymerization on either strand of DNA (leading or lagging strand).
Therefore, the correct option is :leading and lagging
